Physiological and Transcription Analyses Reveal the Regulatory Mechanism in Oat (Avena sativa) Seedlings with Different Drought Resistance under PEG-Induced Drought Stress

Drought severely limits the growth and development of oat (Avena sativa) seedlings. As an osmotic regulator simulating a drought environment, Polyethylene glycol (PEG) has been widely linked in response to plant tolerance. However, underlying mechanism oats’ PEG stress is still largely unknown. Here, we investigated physiological transcriptome variables drought-resistant variety DA92-2F6, drought-susceptible Longyan 3 under 15% PEG-6000 better understand tolerance molecular mechanisms. The results showed that except for cell membrane permeability, antioxidant enzyme, adjustment substance, photosynthetic efficiency were significantly higher DA92-2F6 after 7 d stress. Further, 12 cDNA libraries 123,223 unigenes obtained by RNA-seq. A total 33,857 differentially expressed genes (DEGs) detected, which two co-upregulated three co-downregulated four comparisons. We highlighted analysis DEGs phytohormone signal transduction pathway. auxin, cytokinin, brassinosteroid signaling pathways, suppressed 3, while abscisic acid jasmonic pathways mainly activated upregulated PP2C, ABF, SNRK2, GID1, JAZ, MYC2 may enhance DA92-2F6. Taken together, these provided new transcript resource improvement reference resistance breeding.